4Peaks

The application displays DNA sequencing trace files on macOS, supporting common formats such as AB1 and SCF and overlaying base‑calling quality data on the chromatograms. Users can zoom horizontally or vertically, trim low‑quality regions, and edit traces, with changes saved and exportable as PDF figures for publication.

It provides on‑the‑fly translation of the nucleotide sequence to amino‑acid residues, enabling immediate assessment of mutation effects, and includes a searchable library of over a thousand built‑in sequence motifs. Users can initiate BLAST searches directly from the program and invoke speech synthesis to read the trace aloud.

Designed for molecular biologists, the tool combines visual editing, quality visualization, and quick analytical shortcuts within a native macOS interface, offering plugin support and multi‑language capabilities for routine sequence verification and analysis.